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. 2015 Sep 2;9:310. doi: 10.3389/fnins.2015.00310

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Copyright © 2015 Xing, McDonald, Da Fonte, Gutierrez-Villagomez and Trudeau.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of RGCs in primary culture by in situ hybridization and immunofluorescence. Confocal image of double immunofluorescence labeling of GFAP (red, A) and BLBP (green, B) shows the colocalization of GFAP and BLBP in RGCs in culture (D). Positive staining of cyp19a1b mRNA (E) and aromatase B protein (AromB) in RGC culture (F) indicates the expression of aromatase B in RGC cultures. Confocal imaging of double immunofluorescence labeling of GFAP (red, G) and D1R (green, H) shows the colocalization of GFAP and D1R in RGC culture (J). The nuclear stain DAPI (blue) is also shown (C,I). Scale bar = 10 μm.