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. 2015 Sep 2;9:310. doi: 10.3389/fnins.2015.00310

Figure 6.

Figure 6

Involvement of D1R/cAMP/PKA/p-CREB signaling pathway in dopaminergic regulation of cyp19a1b mRNA in RGC culture. Total cAMP production rapidly increased by 10 μM SKF 38393 (30 min). Data were normalized to control value and defined as % of control, bars represent the mean + SEM (n = 3), and values for each sample were determined in duplicate. (A) Regulation of cyp19a1b mRNA in primary RGC culture after 24 h exposure of 8-Br-cAMP (B), SKF 38393 and/or H89 (C). Data were defined as fold change relative to control, bars represent the mean + SEM of cyp19a1b (n = 4), and values for each sample were determined in duplicate. Western blot image showing the effects of SKF 38393 on p-CREB immunoreactivity, where actin served as internal control (D). The densitometric analysis of western blot is reported as an arbitrary value relative to the average of all bands on the same blot. Data were normalized and defined as fold change relative to control, and bars represent the mean + SEM (n = 6) (E). a,b-Groups marked by different letters are significantly different (P < 0.05), *Groups marked by asterisks are significantly different to control (P < 0.05).