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. 2015 Sep 2;5:13748. doi: 10.1038/srep13748

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(A) Number of CFU-MKs in different mouse groups after incubation with LPS alone or IL-6 with LPS. Colonies classified according to their size were evaluated by inverted light microscopy. Experiments were performed in triplicate in four assays. Significant results compared with PBS are based on *P < 0.05 or **P < 0.01. (B) Bone marrow cells from TPO-pretreated mice were isolated by a discontinuous BSA density gradient and grown in a liquid serum-free medium in LPS alone or with IL-6 for 3 d. Cells were stained for acetylcholinesterase activity and phase contrast morphology of megakaryocyte cells. Scale bar, 10 μm.