Skip to main content
. Author manuscript; available in PMC: 2016 Aug 10.
Published in final edited form as: Dev Cell. 2015 Jul 16;34(3):267–282. doi: 10.1016/j.devcel.2015.05.025

Figure 4. Notch inhibits proliferation and differentiation in a dose-dependent manner.

Figure 4

(A, F) sqet4;sqet20 larvae were treated for 30hrs with the γ-secretase inhibitor LY411575 (LY). After 6hrs in drug, BrdU was added for 24hrs. DMSO treated controls shown in Figures 2B and 2E.

(B-E) Notch inhibition disrupts the proliferative compartments. After 10μM LY, amplifying cell divisions are no longer clustered in the dorso-ventral poles.

(G-J) During regeneration, Notch inhibition enhances differentiating divisions in the central region of the NM and in the normally quiescent anterior and posterior compartments.

(K) Notch inhibition does not affect proliferation rates (BrdU index) during homeostasis.

(L) During regeneration 10μM LY does not affect total proliferation rates but induces differentiation at the expense of amplifying cell divisions. 50μM LY induces hyper-proliferation and an increase in differentiation.

Error bars show the 95% confidence interval (CI).