Table 1.
Comparison of direct and indirect interaction for three registered sections in a case with loose lymphoid infiltrates
| Direct interaction/RBFdirect | Indirect interaction/RBFindirect | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD3 | CD3 Bone | CD4 | CD4 Bone | CD8 | CD8 Bone | CD20 | CD20 Bone | CD3 | CD3 Bone | CD4 | CD4 Bone | CD8 | CD8 Bone | CD20 | CD20 Bone | |
| CD3 | MCD3 = 0.00 | MCD3 = 0.00b | MCD3 = 0.02 | MCD3 = 0.00b | MCD3 = 0.02 | MCD3 = 0.10a | MCD3 = 0.00 | MCD3 = 0.60 | MCD3 = 0.54 | MCD3 = 0.36 | MCD3 = 0.54 | MCD3 = 0.36 | MCD3 = 0.69a | MCD3 = 0.60 | ||
| CD3 Bone | MCD3Bone = 0.00 | MCD3Bone = 0.03 | MCD3Bone = 0.43a | MCD3Bone = 0.00 | MCD3Bone = 0.43b | MCD3Bone=0.00 | MCD3Bone=0.88 | MCD3Bone = 0.89 | MCD3Bone = 0.71 | MCD3Bone = 0.50 | MCD3Bone = 0.71 | MCD3Bone = 0.50 | MCD3Bone=0.59 | MCD3Bone=0.98 | ||
| CD4 | MCD4 = 0.00b | MCD4 = 0.61 | MCD4 = 0.00 | MCD4 = 0.00 | MCD4 = 0.00 | MCD4 = 0.00 | MCD4 = 0.39 | MCD4 = 0.91 | MCD4 = 0.80 | MCD4 = 0.24 | MCD4 = 0.72 | MCD4 = 0.21 | MCD4 = 0.69 | MCD4 = 0.24 | ||
| CD4 Bone | MCD4Bone = 0.00 | MCD4Bone = 0.79a | MCD4Bone = 0.00 | MCD4Bone = 0.00 | MCD4Bone=0.84 | MCD4Bone=0.01 | MCD4Bone=0.31 | MCD4Bone = 0.88 | MCD4Bone = 0.83 | MCD4Bone = 0.36 | MCD4Bone = 0.66 | MCD4Bone=0.92 | MCD4Bone=0.54 | MCD4Bone=0.82 | ||
| CD8 | MCD8 = 0.00b | MCD8 = 0.00 | MCD8 =0.00 | MCD8 = 0.00 | MCD8 = 0.00 | MCD8 = 0.00 | MCD8 = 0.00 | MCD8 = 0.91 | MCD8 = 0.80 | MCD8 = 0.50 | MCD8 = 0.31 | MCD8 = 0.27 | MCD8 = 0.80 | MCD8 = 0.51 | ||
| CD8 Bone | MCD8Bone = 0.00 | MCD8Bone = 0.71b | MCD8Bone = 0.00 | MCD8Bone = 0.96 | MCD8Bone = 0.00 | MCD8Bone=0.01 | MCD8Bone=0.25 | MCD8Bone = 0.89 | MCD8Bone = 0.83 | MCD8Bone = 0.21 | MCD8Bone = 1.00 | MCD8Bone = 0.64 | MCD8Bone=0.51 | MCD8Bone=0.83 | ||
| CD20 | MCD20=0.15 a | MCD20=0.00 | MCD20 = 0.00 | MCD20 = 0.06 | MCD20 = 0.00 | MCD20 = 0.07 | MCD20 = 0.00 | MCD20=0.99 a | MCD20=0.57 | MCD20 = 0.59 | MCD20 = 0.31 | MCD20 = 0.99 | MCD20 = 0.27 | MCD20 = 0.58 | ||
| CD20 Bone | MCD20Bone=0.00 | MCD20Bone=0.91 | MCD20Bone=0.03 | MCD20Bone=0.46 | MCD20Bone=0.00 | MCD20Bone=0.44 | MCD20Bone=0.00 | MCD20Bone=0.90 | MCD20Bone=0.99 | MCD20Bone=0.36 | MCD20Bone=0.50 | MCD20Bone=0.68 | MCD20Bone=0.46 | MCD20Bone=0.60 | ||
Three sequential sections stained for CD3, CD20, CD4 and CD8 were registered and subsequently the overlap coefficients were calculated for direct and indirect interaction (application of RBFdirect with its sharp shape and respectively RBFindirect with its broad silhouette)
aAs expected, small overlap/direct interaction between CD3+ and CD20+ cells and noticeable indirect, paracrine interaction
bUnexpected low overlap between the T cell markers CD3, CD4 and CD8 assumedly due to morphological changes in the course of serial sectioning (see arrows in Additional file 3: Figure S3). Modification of the RBFdirect would overcome this problem, however, at the cost of reduced specificity