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. 2015 Jun 17;6:7491. doi: 10.1038/ncomms8491

Figure 1. Schematic overview of HA gene-deficient influenza virus used for high-throughput screens.

Figure 1

(a) Schematic diagram of influenza virus-like RNA encoding the reporter protein GFP. The GFP coding region is flanked by viral HA sequences that are required for efficient virion incorporation of the vRNA, and by viral regulatory sequences at both ends (indicated by small bars). (b) Generation of HA gene-deficient influenza virus. 293T cells were transfected with plasmids expressing HA, the viral polymerase (PB2, PB1 and PA) proteins and the NP. Cells were co-transfected with plasmids synthesizing all eight vRNAs; the plasmid for the synthesis of wild-type HA was replaced with that encoding GFP (a). Influenza virus encoding GFP can be propagated in MDCK cells expressing HA, but not in normal MDCK cells. (c) Summary of plasmid and virus libraries. AA seq., amino-acid sequence.