Table 2. DNA methylation levels in selected genes for CVID and healthy cohorts in naive, unswitched and switched memory B cells.
| Gene |
Naïve |
Unswitched |
Switched |
|||
|---|---|---|---|---|---|---|
| AVG %Meth | s.d. | AVG %Meth | s.d. | AVG %Meth | s.d. | |
| BCL2L1 | ||||||
| Control | 79 | 22 | 49 | 25 | 13 | 6 |
| CVID | 80 | 31 | 62 | 25 | 21 | 24 |
| TCF3 | ||||||
| Control | 87 | 7 | 42 | 20 | 21 | 10 |
| CVID | 77 | 29 | 62 | 32 | 32 | 25 |
| PIK3CD | ||||||
| Control | 80 | 23 | 33 | 18 | 18 | 16 |
| CVID | 82 | 23 | 54 | 29 | 31 | 26 |
| RPS6KB2 | ||||||
| Control | 87 | 13 | 22 | 28 | 14 | 22 |
| CVID | 76 | 33 | 66 | 40 | 35 | 45 |
| KCNN4 | ||||||
| Control | 93 | 8 | 35 | 28 | 8 | 9 |
| CVID | 86 | 15 | 53 | 37 | 11 | 9 |
| KCNC4 | ||||||
| Control | 87 | 7 | 40 | 20 | 11 | 8 |
| CVID | 84 | 17 | 41 | 41 | 59 | 37 |
| CORO1B/PTPRCAP | ||||||
| Control | 81 | 18 | 46 | 38 | 26 | 25 |
| CVID | 87 | 11 | 72 | 31 | 64 | 39 |
| Control | 81 | 31 | 21 | 29 | 18 | 23 |
| CVID | 88 | 15 | 44 | 40 | 31 | 38 |
Avg, average; CVID, common variable immunodeficiency; Meth, DNA methylation.
DNA methylation levels are measured by bisulfite pyrosequencing and represented as percentage.