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. 2015 Jun 17;6:7474. doi: 10.1038/ncomms8474

Figure 3. Antigen-specific T-cell responses were attenuated in T-Red mice.

Figure 3

(a,b) Collagen-induced arthritis model. Clinical scores (a) and serum concentrations of IL-17 (b) in T-Red and control mice (5–8 weeks old). Serum IL-17A concentrations were measured by ELISA before antigen immunization (unimmunized), 21 days after immunization (d21) and 6 days after secondary immunization on day 21 (d21+6). (c,d) T-cell dependent response to OVA. Serum concentrations of the anti-OVA IgM (c) and anti-OVA IgG1 (d) were measured in T-Red and control mice (7–9 weeks old) after immunization with OVA in the presence of alum. (e,f) Male cell rejection in female mice. Congenic CD45.1 male (e) or female (f) splenocytes were transferred into female T-Red or control mice (6–8 weeks old). Percentages of the transferred donor cells in peripheral blood (PBL) are shown. (g,h) Bacteria infection. Listeria monocytogenes expressing OVA was infected in T-Red and control mice (6–8 weeks old). Cell numbers of OVA-specific IFNγ+ populations in CD8+ T cells on day 7 post infection are shown (g). The same number (20,000 cells) of OT-I or T-Red/OT-I cells was transferred into WT congenic hosts 1 day before infection. The frequency of donor cells in the blood CD8+ T cells was determined on day 7 post infection (h). Data represent the mean±s.e.m. (a, n=8–10; c–f, n=4; and g–h, n=4–5). Wilcoxon test was used in a. *P<0.05; **P<0.01; ***P<0.001. NS, not significant.