Figure 1. miR-155 is transferred between BMDCs and is present in exosomes.

(a) A schematic of the co-culture experiment. (b) Representative FACS plots where co-cultured CD45.1⁺ Wt and CD45.2⁺ miR-155−/− CD11c⁺ BMDCs were separated (n=4). (c) Relative miR-155 levels were quantified via qRT–PCR from isolated miR-155−/− BMDCs that had been cultured alone or with Wt BMDCs in the presence or the absence of LPS for 24 h (n=4). (d) Relative miR-155 levels were measured via qRT–PCR in miR-155−/− BMDCs either cultured alone or with Wt BMDCs separated by a 0.4-μm filter for 24 h with or without LPS (n=3). (e) Cryo-EM of exosomes isolated from Wt BMDCs. Scale bar, 200 nm. Red box is enlarged in the upper left corner. (f) CD63 protein levels in the exosomal pellet from Wt and miR-155−/− BMDCs treated with or without LPS. (g) Relative levels of miR-155 in exosomes derived from Wt or miR-155−/− BMDCs treated with or without LPS (n=3). (h) Exosome quantification of Wt BMDCs treated with or without GW4869 (n=3). Limit of detection is 2 × 10⁷ exosomes. (i) Relative levels of miR-155 were measured in the exosomal pellet from Wt BMDCs treated with or without LPS and GW4869 as quantified by qRT–PCR (n=2). (j) Exosome quantification of Wt and Rab27 DKO BMDC-derived exosomes (n=2). Limit of detection is 2 × 10⁷ exosomes. (k) miR-155 levels in exosome pellets from Wt and Rab27 DKO BMDC-conditioned medium (n=2). Data represent two independent experiments and are presented as the mean±s.d. (error bars). *P<0.05; **P<0.01, Student's t-test.