. 2015 Sep 2;14:39. doi: 10.1186/s12941-015-0102-4

Table 2.

Primers used in this study for amplification of class 1 and class 2 integrons and other antibiotic resistant genes

Primer pair	Target	Sequence (5´–3´)	Annealing temperature (◦C)	Amplicon size (bp)	References
Primer 1 16s-8F	16S rDNA	AGAGTTTGATCMTGGCTCAG	60	456	[36]
Primer 2 16s-517	16S rDNA	ATTACCGCGGCTGCTGG	60	456	[37]
IntI1_F	Class 1 integrase gene	CCTCCCGCACGATGATC	55	270	[38]
IntI1_R	Class 1 integrase gene	TCCACGCATCGTCAGGC	55	270	[38]
IntI2_F	Class 2 integrase gene	TTATTGCTGGGATTAGGC	50	233	[39]
IntI2_R	Class 2 integrase gene	ACGGCTACCCTCTGTTATC	50	233	[39]
5′_CS	Class 1 integron variable region	GGCATCCAAGCAGCAAG	58.5	Variable	[40]
3′_CS	Class 1 integron variable region	AAGCAGACTTGACCTGA	58.5	Variable	[40]
Hep_F	Class 2 integron variable region	CGGGATCCCGGACGGCATGCACGATTTGTA	60	Variable	[41]
Hep_R	Class 2 integron variable region	GATGCCATCGCAAGTACGAG	60	Variable	[41]
Sul1-F	sul1	CGGCGTGGGCTACCTGAACG	60	433	[42]
Sul1-R	sul1	GCCGATCGCGTGAAGTTCCG	60	433	[42]
Sul2-F	sul2	GCGCTCAAGGCAGATGGCATT	60	293	[42]
Sul2-R	sul2	GCGTTTGATACCGGCACCCGT	60	293	[42]
pVP440sul3F	sul3	TCAAAGCAAAATGATATGAGC	55	787	[42]
pVP440sul3R	sul3	TTTCAAGGCATCTGATAAAGAC	55	787	[42]
qacEΔ1F	qacEΔ1F	ATC GCA ATA GTT GGC GAA GT	58	800	[43]
sul1-B	sul1-B	GCA AGG CGG AAA CCC GCG CC	58		[44]
aph (3″)^c-F	aph (3″)^c	GCTCAAAGGTCGAGGTGTGG	55	515	[27]
aph (3″)^c-R	aph (3″)^c	CCAGTTCTCTTCGGCGTTAG	55	515	[27]
ant (3″)^b-F	ant (3″)^b	CAGCGCAATGACATTCTTGC	55	295	[27]
ant (3″)^b-F	ant (3″)^b	GTCGGCAGCGACA(C/T)CCTTCG	55	295	[27]
aph(6)-1d^d-F	aph(6)-1d^d	GACTCCTGCAATCGTCAAGG	55	560	[27]
aph(6)-1d^d-R	aph(6)-1d^d	GCAATGCGTCTAGGATCGAG	55	560	[27]
tet(A)-F	tet(A)	TTGGCATTCTGCATTCACTC	60	494	[45]
tet(A)-R	tet(A)	GTATAGCTTGCCGGAAGTCG	60	494	[45]
tet(B)-F	tet(B)	CAGTGCTGTTGTTGTCATTAA	60	571	[45]
tet(B)-R	tet(B)	GCTTGGAATACTGAGTGTAA	60	571	[45]
tet(E)-F	tet(E)	TATTAACGGGCTGGCATTTC	55	544	[45]
tet(E)-R	tet(E)	AGCTGTCAGGTGGGTCAAAC	55	544	[45]
tet(M)-F		ACACGCCAGGACATATGGAT	55	536	[45]
tet(M)-R	tet(M)	ATTTCCGCAAAGTTCAGACG	55	536	[45]
tet(30)-F	tet(30)	CCGTCATGCAATTTGTGTTC	55	550	[45]
tet(30)-R	tet(30)	TAGAGCACCCAGATCGTTCC	55	550	[45]
SHV_F	bla _SHV	GCGAAAGCCAGCTGTCGGGC	62	538	[46]
SHV_R	bla _SHV	GATTGGCGGCGCTGTTATCGC	62	538	[46]
CTX_F	bla _CTX-M	GTGCAGTACCAGTAAAGTTATGG	55	538	[46]
CTX_R	bla _CTX-M	CGCAATATCATTGGTGGTGCC		538	[46]
TEM_F	bla _TEM	AAAGATGCTGAAGATCA	44	425	[46]
TEM_R	bla _TEM	TTTGGTATGGCTTCATTC		425	[46]