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. 2015 Aug 14;6:8042. doi: 10.1038/ncomms9042

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(a) Schematic representation of the experimental setup showing a supported lipid bilayer with BaxG bound to it. (b) Epifluorescence image of BaxG molecules immobilized on a supported lipid bilayer. Scale bar, 1 μm. Each particle was detected and the fluorescence intensity measured over time. Detected particles are shown in circles to help visualization. (c,d) Fluorescence intensity of two representative individual particles showing photobleaching steps (arrow). (e) Histogram of the fluorescence intensity distribution of individual BaxG particles with a single bleaching event, fitted with a Gaussian to obtain the fluorescence intensity of a single fluorophore. (f) Mean fluorescence intensity (μ) and s.d. (σ) values calculated for individual particles containing one (orange), two (green), three (blue), four (magenta), five (cyan) and six (red) fluorophores.