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. 2015 Aug 14;6:8042. doi: 10.1038/ncomms9042

Figure 2. BaxG binds as a monomer to membranes containing CL in presence of cBid.

Figure 2

Binding of BaxG particles to SLBs is negligible for membranes composed of phosphatidylcholine:cardiolipin (PC:CL) (8:2) in absence of cBid (a) and for membranes composed of pure PC in presence of cBid (b), but it is very efficient for membranes composed of PC:CL (8:2) in presence of cBid (c). The images show similar scaling to compare them visually. Scale bar, 1 μm. (d) Fluorescence intensity distribution of single BaxG particles added to SLBs made of PC:CL (8:2) in presence of cBid. Approximately 500 particles were analysed. The resulting histograms were fitted with a linear combination of four Gaussians to estimate the occurrence of particles containing one (orange), two (green), three (blue) and four (magenta) labelled molecules. The cumulative fit is shown in black. The area of the fitted Gaussians is proportional to the fraction of each species. (e) Percentage of BaxG monomers, dimers, trimers and tetramers calculated from the averaged distributions of species from three different experiments, after correction for partial labelling. The error bars correspond to the average error for each species.