Fig. 4.

Early intervention with anti-Fc receptor I for immunoglobulin (Ig)A (FcαRI) monoclonal antibody inhibits renal inflammation and macrophage infiltration in lupus-associated glomerulonephritis induced by pristane. (a) Histological analysis [periodic acid-Schiff (PAS) and Masson's trichrome (MT) stain] and immunohistological (IHC) analysis (F4/80 and fibronectin) of kidney sections from transgenic (Tg) mice (magnification: ×400). (b) Mesangial immunoglobulin and complement deposition were observed in the control groups treated with normal saline (G2P) or control antibody (G4P) by fluorescent microscopy (magnification: ×200). Macrophage inflammatory protein (MIP)-8a-treated group (G3P) showed less deposition. Green = fluorescein isothiocyanate (FITC); red = rhodamine. The MIP-8a-treated group (G3P) had a lower glomerular activity index (c) and fewer F4/80-positive macrophages in glomeruli (d). Of note, the glomerular activity index and number of F4/80-positive macrophages in glomeruli obtained for G1P were zero. Non-normally distributed data are presented as the median (Q25, Q75) (n = 3 for G2P, G3P, G4P; n = 4 for G1P), and were analysed using the Kruskal–Wallis test with Bonferroni's method for adjusting the alpha (P < 0·017 is considered statistically significant). ^#P < 0·017 G3P versus G2P; ^ΔP < 0·017 G4P versus G3P.