Fig. 2.

Whole blood cell counts in simian varicella virus (SVV) latently infected animals undergoing immunosuppressive treatment. Absolute numbers of lymphocytes (closed square), white blood cells (open square), neutrophils (open triangle) were counted per ml of whole blood at various days post-infection in (a) cohort 1, (c) cohort 2 and (e) cohort 3. Absolute number of CD4 T cells (open circle), CD8 T cells (closed square) and CD20 B cells (closed triangle) were calculated per ml of whole blood from flow cytometric analysis using the lymphocyte count in (b) cohort 1, (d) cohort 2 and (f) cohort 3. Average ± standard error of the mean (s.e.m.). Dashed line indicates onset or change in immunosuppressive treatment; total body irradiation (TBI), cyclosporin A (CsA), tacrolimus (TAC), prednisone (pred), anti-thymocyte globulin (ATG), anti-CD3 immunotoxin (CD3-IT) and Janus activated kinase inhibitor (JAK-Inh). Statistical analysis was performed using one-way repeated-measures analysis of variance (anova) with Tukey's post-test to test differences between pre- and post-treatment values P < 0·001 ^XXXlymphocyte, ***CD4 T cell, ^###CD8 T cell, ⁺⁺⁺CD20 B cell; P < 0·01 ^&&white blood cell, ^**CD4 T cell, ^##CD8 T cell; P < 0·05 and white blood cell, ^$neutrophil, ^#CD8 T cell.