Expression profiles of CBR and related genes in Z. tritici. (A) Mean FPKM values showing expression of Z. tritici CBRs in Czapek Dox broth (CDB), potato dextrose broth (PDB) and at one, four, nine, 14 and 21 days post inoculation (DPI) of wheat leaves; significant differences in expression of ZtCBR2 and ZtCBR3 (* p < 0.05) were found during growth in PDB relative to during growth in CDB. (B) Mean FPKM values showing expression profile of ZtCBR2 and the two neighbouring genes, ZtCYP-24 and a putative hydroxyacyl CoA dehydrogenase across the same set of conditions. (C) A diagram showing gene organisation across the region with the total size of the putative three gene cluster in base pairs indicated below. The two genes neighbouring ZtCBR2 were significantly up-regulated both in PDB and on day four of infection (* p < 0.05) relative to during growth in CDB; ZtCBR2 exhibited a similar expression profile though apparent up-regulation was only significant in PDB (p < 0.05).