Fig. 1.

Phenotypic analyses of chs2Δchs8Δ mutant yeast and hyphae. A–D. Yeast cells of the chs2Δchs8Δ mutant and wild-type (CAI-4) strains were grown in SD broth at 30 °C for 24 h and counterstained with PI. Panels show representative images of PI-positive stained small buds in the chs2Δchs8Δ mutant (A and B), PI-positive stained large buds in wild-type cells (C), and PI-positive stained mother-and-bud in wild-type cells (D). E–I. Hyphae of the chs2Δchs8Δ mutant were grown on 20% FCS agar pads with PI at 37 °C for 4 h. Panels show representative images of the PI-positive phenotypes indicative of dead mother cells (E), dead buds (F), dead short hyphae (G), dead end compartments (H) and dead mid-compartments (I). All panels show the overlay of PI fluorescence on the corresponding DIC image. Scale bars represent 5 μm.