Fig. 2.

Localization of Chs2-YFP and Chs8-YFP in yeast and hyphal cells. Chs2-YFP was imaged in yeast and hyphae of the CHS2-YFP/chs2Δ0 strain (A–G) and Chs8-YFP was imaged in yeast of the CHS8-YFP strain (H). A. Single snap-shot images of yeast cells with different sized buds grown in YPD at 30 °C for 4 h and then counterstained with CFW. Chs2-YFP localized to the tip of a small bud (first panel), and as a bar (third panel) and spot (fourth panel) at septa of cells where the mother and bud are approximately equal in size. B. Selected frames of a time-lapse movie of Chs2-YFP in a yeast cell grown on an SC agar pad at 30 °C. Images were recorded every 3 min. C. Single snap-shot images of a hypha grown on an agar pad containing 20% FCS and CFW at 37 °C for 6 h showing the persistent localization of Chs2-YFP at hyphal septa and at hyphal tips (yellow arrow). D. Selected frames of a time-lapse movie of Chs2-YFP in a hypha grown on a 20% FCS agar pad at 37 °C. Images were recorded every 3 min. E. Single snap-shot image of a hypha grown on an agar pad containing 20% FCS and CFW at 37 °C for 6 h showing the persistent localization of Chs2-YFP at hyphal septa (white arrows). F–G. Higher magnification image showing Chs2-YFP localized as two dots (G) on either side of a hyphal septum stained with CFW (G). H. Single snap-shot images of yeast cells with different sized buds grown in YPD at 30 °C for 4 h showing Chs8-YFP localized at septation sites as a bar (third panel) and as a dot (fourth panel) at septa. DIC (B – left panels, D – left panels, H – top panels), YFP (A – bottom panels, B – right panels, C, D – right panels, E, F, H – bottom panels), CFW (A top panels), merge YFP and CFW (G). Scale bars represent 5 μm.