Table 2.
Percentage of PI-positive stained yeast cells after growth in SD broth for 24 h at 30 °C.
| Strain | Small buda | Large budb | Both mother-and-bud and unbudded cellsc | Total ± SD |
|---|---|---|---|---|
| wild-type (CAI-4) | 0.00 | 2.33 | 1.38 | 3.71 ± 1.9 |
| chs2Δ | 2.88 | 0.92 | 1.83 | 5.62 ± 0.9 |
| chs8Δ | 0.38 | 0.14 | 1.83 | 2.35 ± 0.4 |
| chs2Δchs8Δ | 3.94 | 2.99 | 2.35 | 9.28 ± 5.2 |
The percentage is the average of two independent biological replicates (n = 250–750 per replicate). Errors are SD.
a
Where the bud is less than one fourth of the size of the mother cell (Fig. 1A and B).
b
Where the bud is greater than one fourth of the size of the mother cell (Fig.1C).
c
Where the bud and mother are PI-positive stained cells (counted as two cells) (Fig. 1D).