Table 5.
Distribution of PI-positive phenotypes in hyphae after 4 h and 24 h incubation at 37 °C in the presence of a near-MIC concentration of caspofungin.
| Phenotype | Time | Strain |
|||
|---|---|---|---|---|---|
| wild-type (CAI-4) + CAS |
chs2Δchs8Δ + CAS |
||||
| Counts | % | Counts | % | ||
| Dead mothersa | 4 h | 48 | 11.40 | 28 | 5.13 |
| Dead budsb | 23 | 5.46 | 25 | 4.58 | |
| Dead short hyphaec | 92 | 21.85 | 297 | 54.40∗ | |
| Dead end compartmentsd | 13 | 3.09 | 14 | 2.56 | |
| Dead mid-compartmentse | 0 | 0.00 | 0 | 0.00 | |
| Lysed tipsf | 31 | 7.36 | 7 | 1.28∗ | |
| Live yeast | 10 | 2.38 | 12 | 2.20 | |
| Live hyphae | 204 | 48.46 | 163 | 29.85∗ | |
| Total | 421 | 100.00 | 546 | 100.00 | |
| Dead mothersa | 24 h | 60 | 7.98 | 31 | 6.07 |
| Dead budsb | 22 | 2.93 | 17 | 3.33 | |
| Dead short hyphaec | 66 | 8.78 | 241 | 47.16∗ | |
| Dead end compartmentsd | 20 | 2.66 | 38 | 7.44 | |
| Dead mid-compartmentse | 36 | 4.79 | 5 | 0.98 | |
| Lysed tipsf | 63 | 8.38 | 5 | 0.98∗ | |
| Live yeast | 5 | 0.66 | 5 | 0.98 | |
| Live hyphae | 480 | 63.83 | 169 | 33.07 | |
| Total | 752 | 100.00 | 511 | 100.00 | |
aFig. 1E, bFig. 1F, cFig. 1G, dFig. 1H, eFig. 1I, fFig. 5D and E. Counts are the total number of hyphae observed displaying a particular phenotype in three independent biological replicates. % is the count expressed as a percentage of the total number of cells counted. ∗ Indicates a statistically significant difference in the proportion of cells displaying a phenotype between the two strains grown in the presence of caspofungin (P ⩽ 0.05 by Mann–Whitney U test).