Table 2. Clonotypic analysis of CD8 T cells specific for the PPI15-24 epitope.
| Subject | TRBV | CDR3 | TRBJ | Frequency (%) |
|---|---|---|---|---|
| Control 2 | 25-1 | CASSDLQAGQPQH | 1-5 | 81.48 |
| 20-1 | CSASVAGEQF | 2-1 | 16.67 | |
| 25-1 | CASSDGQAGQPQH | 1-5 | 1.85 | |
| Control 28 | 10-1 | CASSEFRRWNYGYT | 1-2 | 71.74 |
| 30 | CAWSVNFYINEQF | 2-1 | 28.26 | |
| Patient 25 | 20-1 | CSARDLLWTSGEETQY | 2-5 | 75.29 |
| 2 | CASRPGTGGINEQF | 2-1 | 7.06 | |
| 9 | CASSDYGRGANVLT | 2-6 | 4.71 | |
| 29-1 | CSVQGTGAYEQY | 2-7 | 3.53 | |
| 4-3 | CASSHDADGYT | 1-2 | 3.53 | |
| 14 | CASSPTDRGRGNTEAF | 1-1 | 2.35 | |
| 9 | CASSDFQGAGNTIY | 1-3 | 2.35 | |
| 10-3 | CAISWDRRTYEQY | 2-7 | 1.18 | |
| Patient 26 | 9 | CASSGGWREQF | 2-1 | 71.60 |
| 10-3 | CAISDGDNSHGYT | 1-2 | 16.05 | |
| 27 | CASSLTGTSSYEQY | 2-7 | 8.64 | |
| 7-7 | CASSTYRGRVSLDEQF | 2-1 | 2.47 | |
| 7-7 | CASSTYRGRVSLEEQF | 2-1 | 1.23 | |
| Patient 4 | 27 | CASSPTPSTYNEQF | 2-1 | 20.83 |
| 5-1 | CASSFRTGESYEQY | 2-7 | 16.67 | |
| 5-6 | CASSLGVFGTPSYEQY | 2-7 | 10.42 | |
| 12-3/12-4 | CASSPYGGRNGELF | 2-2 | 10.42 | |
| 30 | CAWALFGSAYEQY | 2-7 | 6.25 | |
| 29-1 | CSVDAPWSSSTDTQY | 2-3 | 4.17 | |
| 7-2 | CASSFYVTGNTEAF | 1-1 | 4.17 | |
| 30 | CAWSVGAGNGYT | 1-2 | 4.17 | |
| 18 | CASSIEVGYEQY | 2-7 | 4.17 | |
| 7-2 | CASSGTGGSYEQY | 2-7 | 4.17 | |
| 5-1 | CASSLAGQGANYGYT | 1-2 | 2.08 | |
| 12-3/12-4 | CASSSRDRVTDTQY | 2-3 | 2.08 | |
| 18 | CASSPSGVRQPQH | 1-5 | 2.08 | |
| 7-2 | CASSLAIGNTEAF | 1-1 | 2.08 | |
| 5-1 | CASSWDRVYNEQF | 2-1 | 2.08 | |
| 2 | CAIPGTALNEQF | 2-1 | 2.08 | |
| 12-3/12-4 | CASSTTDTQY | 2-3 | 2.08 |
Individual clonotypes are represented in order of relative frequency. Gene usage and CDR3 amino acid sequence are shown in each case.