Figure 4.

Host CD103⁺ dendritic cell differences in IL-12/23 production can affect the proportions of pathogenic and regulatory T cells. (a) IL-23 and IL-6 mRNA expression by unstimulated (not sure we can say it is unstimulated because they are after T cell transfer, only if you want to emphasize it is not stimulated in vitro, maybe it is not necessary) FACS sorted MHCII^highCD11c⁺CD103⁺ DCs freshly isolated from the MLNs of B6 and B10-RAG2^−/− mice 2 weeks following adoptive transfer of CD4⁺CD25CD45RB^high T cells. Data shown are mean ± SD of 2 independent experiments (IL-23p19) with pooled cells from 10 mice per group in each; and mean ± SD of Ct triplicates (IL-6) representative of 3 independent experiments with 10 mice per group in each. (b) Serum IL-12p70 and IL-23 following intraperitoneal injection of LPS. Results represent mean ± SD of 3 mice per group from 2 independent experimets (IL-23), and mean ± SD of five mice per group from at least 3 independent experiments (IL-12p70). (c) Study design of anti-IL-12/23p40 treatment. (d) Flow cytometry analysis of lymphocytes, gated based on characteristic light-scatter properties, isolated from the MLNs of B6 and B10-RAG2^−/− recipients of CD4⁺CD25CD45RB^high T cells treated with intraperitoneal injection of 1.5mg of neutralizing anti-IL-12/23p40 or control IgG. Data shown are representative of 2 independent experiments with similar results. Cells for analysis are pooled from 5 mice per group per experiment. Asterisks indicate statistically significant difference between the two strains (**P<0.01, ***P<0.001).