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. Author manuscript; available in PMC: 2015 Sep 2.
Published in final edited form as: Mol Microbiol. 2009 Sep 3;74(1):159–174. doi: 10.1111/j.1365-2958.2009.06858.x

Fig. 4.

Fig. 4

Analysis of amyA transcript levels. (A) Serotype M1 strain MGAS5005 was grown to early-exponential (exp), mid-exponential, late-exponential, and stationary growth phase in indicated media in quadruplicate biologic replicates on three separate occasions (total of 12 samples at each time point). RNA was isolated, converted to cDNA, and transcript levels for amyA and the endogenous control gene proS were determined using TaqMan real-time PCR. Data graphed are mean +/− standard deviation. (B) amyA transcript levels for 6 patients with GAS pharyngitis were determined by TaqMan real-time PCR. The M serotype of the infecting GAS strain is shown in the circle. Data graphed are mean +/− standard deviation with each experiment done with quadruplicate technical replicates on three separate occasions (total of 12 data points per sample).