Table 1.
Strains and plasmids.
| Strain or plasmid | Description | Genotype | Reference |
|---|---|---|---|
| Strains | |||
| MGAS5005 | Invasive isolate, serotype M1 | ΔcovS nt80 | (Sumby et al., 2005) |
| MGAS5005(pDC123) | MGAS5005 with empty vector | MGAS5005, Cmr | (Shelburne et al., 2006) |
| 5005.100 | ΔamyA strain | MGAS5005 amyA::spc | This study |
| 5005.100(pDC123) | ΔamyA strain with empty vector | ΔamyA, Spcr Cmr | This study |
| 5005.100(pSS100) | ΔamyA strain with complementation vector | ΔamyA/amyA+, Spcr Cmr | This study |
| 5005.200 | ΔpulA strain | MGAS5005 pulA::spc | This study |
| MGAS294 | Invasive isolate, serotype M1 | V286F in covS | (Sumby et al., 2006) |
| MGAS315 | Invasive isolates, serotype M3 | Wild-type | (Beres et al., 2002) |
| MGAS2217 | Invasive isolate, serotype M1 | 1 bp insert in covS | (Sumby et al., 2006) |
| MGAS2221 | Pharyngeal isolate, serotype M1 | Wild-type | (Sumby et al., 2006) |
| MGAS5322 | Pharyngeal isolate, serotype M1 | Wild-type | (Sumby et al., 2006) |
| MGAS5392 | Pharyngeal isolated, serotype M1 | Wild-type | (Sumby et al., 2006) |
| MGAS6184 | Invasive isolate, serotype M1 | A397V in covS | (Sumby et al., 2006) |
| MGAS9506 | Pharyngeal isolate, serotype M | Wild-type | (Sumby et al., 2006) |
| Klebsiella oxytoca M5a1 | Wild-type | (Fiedler et al., 1996) | |
| Plasmids | |||
| pSL60-1 | Vector containing aad9 gene | Spcr | (Lukomski et al., 2000) |
| pDC123 | Chloramphenicol resistance vector | Cmr | (Chaffin & Rubens, 1998) |
| pSS100 | amyA complementation vector | Cmr | This study |
| pBAD_amyA | pBAD vector with mature amyA sequence | Ampr | This study |