Fig. 10.

Cytoplasmic mislocalization of PSF, reduction of NMHC II-B, and abnormal splicing of NMHC II-B in the brains of ALS patients. a Double immunofluorescent staining of the cortex and spinal cord from the ALS patient and non-ALS individual (control) with antibodies to PSF and NeuN. Scale bars: cortex: 10 μm. Spinal cord: 50 μm. Note that ALS patient brain shows cytoplasmic distribution of PSF and NeuN. b Immunostaining of the brain cortex in control and ALS patients with single anti-PSF only revealed PSF immunofluorescent staining. c Quantitative analysis of the percentage of cells with cytoplasmic PSF and NeuN staining. The staining of the nuclear protein TFIIB served as controls. The data are mean ± SE by counting 60 to 80 cells per sample (n = 3per group). *p < 0.05, **p < 0.01, ***p < 0.001