Fig. 6.

Interactions of TDP-43 with PSF and NeuN. a Representative western blots showing co-immunoprecipitation of transfected flag-TDP-43 (WT or mutant TDP-43 (M337V)) by anti-flag and endogenous PSF, NeuN in mouse N2A cells. IgG immunoprecipitation served as controls. The ratios of precipitated to input were obtained from 3 independent experiments and shown beneath the blots. b The in vivo interaction of TDP-43 (M337V) with PSF and NeuN in the non-transgenic (C) pig and transgenic TDP-43 (TG) pig brain. The immunoprecipitation was also pre-treated with RNase to remove RNA. An antibody to TDP-43 (12892-1-AP from Proteintech) selectively detected human TDP-43, but not endogenous pig TDP-43. The interaction of FUS with TDP-43, which depends on RNA, served as a control. Quantitative data (ratio of IP/input) are presented beneath the blots. IP bands indicated by arrows were used for quantification. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with –RNase treatment