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. 2015 Jul 14;34(16):2117–2131. doi: 10.15252/embj.201490315

Figure 3.

Figure 3

Induction of STE and TAG enzymes rescues autophagy

  • A GAL-DGA1 GAL-ARE2 lro1Δ are1Δ (FYS118 strain) was grown on SC (synthetic minimal medium without dextrose) + raffinose overnight, diluted to OD 0.4, and grown to mid-log phase in either SC + glucose or SC + galactose medium. Cells were shifted to SD-N for 2 h, stained with BODIPY, and visualized by fluorescence microscopy. Scale bar, 5 μm.
  • B WT (BY4741) and GAL-DGA1 GAL-ARE2 lro1Δ are1Δ (FYS118) cells expressing GFP-Atg8 were grown as in (A). Cells were lysed at the indicated times and subjected to SDS–PAGE, followed by Western blot analysis using anti-GFP antibodies.
  • C, D WT (BY4741) and GAL-DGA1 GAL-ARE2 lro1Δ are1Δ (FYS118) cells expressing GFP-Atg8 (C) or GFP-Atg1 (D) were grown as in (A) and visualized by fluorescence microscopy after 2 h in SD-N. Scale bar, 5 μm.

Data information: DIC, differential interference contrast; SD-N, nitrogen starvation medium; WT, wild type. Source data are available online for this figure.