Figure 2.
Validation of PP2A-C as a mediator of cisplatin cytotoxicity. A, Western blot showing stable knockdown of PP2A-C in OVCAR-8 cells. B, Cell viability (MTT) assay demonstrating increased sensitivity to cisplatin and LB100 following PP2A-C knockdown. C and D, Western Blot showing hyperphosphorylation of Chk1 (S345) for up to 8 hrs following cisplatin washout in PP2A-C knockdown (C) and LB100 treated (D) OVCAR-8 cells.