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. Author manuscript; available in PMC: 2015 Sep 2.
Published in final edited form as: ACS Chem Biol. 2013 Jul 16;8(9):1947–1954. doi: 10.1021/cb400259n

Figure 3.

Figure 3

Uptake of FFN206 in cultured cells is dependent on VMAT2 activity: Epifluorescence images of HEK cells (VMAT2 transfected and null-transfected controls) treated with FFN206 (5 µM, 2 hr incubation) in the absence or presence of TBZ (2 µM, 1 hr preincubation followed by coincubation with the probe). (A) FFN206 selectively accumulates in cells expressing VMAT2. (B) Negligible uptake of FFN206 in VMAT2-HEK cells pre-treated with TBZ. (C) HEK293 cells (not expressing VMAT2) pre-treated with DMSO vehicle. (D) Control HEK293 cells pre-treated with TBZ also accumulate negligible amounts of FFN206. Top row = epifluorescence images, bottom row = brightfield images.