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. Author manuscript; available in PMC: 2015 Sep 2.
Published in final edited form as: ACS Chem Biol. 2013 Jul 16;8(9):1947–1954. doi: 10.1021/cb400259n

Figure 6.

Figure 6

A selected collection of drugs was screened for VMAT2 inhibition in FFN206 uptake assay. The broad range of inhibition effects can be measured in HTS format. The cells were first incubated with 180 µl experimental medium with addition of 10 µl inhibitor (20× the final concentration) for 30 min, followed by addition of 10 µl FFN206 (20 µM) and incubated for 60 min. The final concentrations of tested compounds are 10 µM except reserpine for which 1 µM was used. The final concentration for FFN206 is 1 µM. The uptake was terminated with one PBS buffer wash. Error bars are standard errors (S.E.) derived from 4 independent experiments. Each experiment was run in triplicate per plate and 2 plates total.