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. Author manuscript; available in PMC: 2016 Aug 24.
Published in final edited form as: Dev Cell. 2015 Aug 24;34(4):400–409. doi: 10.1016/j.devcel.2015.08.001

Fig. 1. PI(4)P and PI(4,5)P2 are present in distinct ciliary compartments.

Fig. 1

(A) IMCD3 cells transfected with EGFP-2×P4MSidM, a PI(4)P sensor, were stained with antibodies against the ciliary protein Arl13b (red) and EGFP (green). Nuclei were marked by DAPI (blue). (B) Normalized EGFP-2×P4MSidM intensity for ten IMCD3 cilia was plotted against normalized distance along the cilium. The black dotted line is the linear regression of all cilia. (C) Cilia of live NIH-3T3 cells were visualized by 5HT6-CFP fluorescence (false colored red). PHPLCd1- EYFP, a PI(4,5)P2 sensor, accumulated in the proximal ciliary region (false colored green). Scale bar, 5μm. (D) Normalized EYFP-PHPLCd1 intensity for eleven NIH-3T3 cilia was plotted against normalized distance along the cilium and the data fitted to sigmoidal curves. The black dotted line is the average of all curves.