Table 2.
Down-regulated transcripts in zfs1 Δ/Δ mutants.
| Orf name | Gene | Change | P value | 9-mers | 7-mers | RPKM | Function |
|---|---|---|---|---|---|---|---|
| orf19.5334 | ZFS1 | −12 324.8 | 6.4E−185 | 0 | 4* | 975.1 | Ortholog of Saccharomyces cerevisiae Cth2, an mRNA-binding protein |
| orf19.7585 | INO1 | −2.3 | 5.1E−06 | 0 | 0 | 8.2 | Inositol-1-phosphate synthase |
| orf19.1344 | −2.3 | 4.4E−04 | 0 | 0 | 90.8 | Protein of unknown function | |
| orf19.6993 | GAP2 | −2.3 | 3.4E−05 | 0 | 0 | 8.0 | General amino acid permease |
| orf19.6073 | HMX1 | −2.2 | 1.8E−06 | 0 | 0 | 61.1 | Heme oxygenase |
| orf19.1415 | FRE10 | −2.1 | 8.7E−11 | 0 | 0 | 116.1 | Major cell-surface ferric reductase under low-iron conditions |
| orf19.2602 | OPT1 | −2.1 | 5.0E−08 | 0 | 0 | 88.6 | Oligopeptide transporter |
| orf19.4765 | PGA6 | −2.1 | 1.5E−06 | 0 | 0 | 142.8 | GPI-anchored cell wall adhesin-like protein |
| orf19.6570 | NUP | −2.1 | 1.6E−03 | 0 | 0 | 9.4 | Nucleoside permease |
| orf19.7276.1 | TLO4 | −2.0 | 9.9E−03 | 0 | 0 | 30.4 | Member of a family of telomere-proximal genes |
| orf19.4716 | GDH3 | −2.0 | 9.3E−05 | 0 | 0 | 297.0 | NADP-glutamate dehydrogenase |
Shown are down-regulated transcripts with average fold changes, P values, average RPKM (reads per kilobase of transcript per million reads mapped) for wild type (WT) and proposed function for two independent isolates of zfs1ΔΔ mutants versus WT as determined by mRNA-Seq. The number of 9-mers and 7-mers within the predicted 3′ UTR is also shown. The asterisk (*) indicates overlapping 9-mers or 7-mers.