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. 2015 Sep 2;10(9):e0137664. doi: 10.1371/journal.pone.0137664

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 1 — (A) Western blot to show GPC3 knockdown efficiency in Hep3B cells and Huh-7 cells. (B) Wound healing assay to detect cell migration ability in GPC3 knocked down Hep3B cells and Huh-7 cells. Scale bar indicates 400 μm. (C) Trans-well assay to examine cell motility in wild type and GPC3 knocked down cells (sh2). Scale bar indicates 50 μm. The OD_590nm value of wild type group was set up as 100%. Values represent mean ± SD from three replicates. P**<0.01.