Table 3. The highest values of each enzyme activity and the occurrence time during the bioconversion with different amino acids as substrates in the presence of glucose.
Values are the means of three replications and shown with standard deviation.
| Substrate | PAL (U/mg) | Time (h) | TAL (U/mg) | Time (h) | C4H (U/mg) | Time (h) | 4CL (U/mg) | Time (h) |
|---|---|---|---|---|---|---|---|---|
| Phe | 390.0±11.0 a | 32 | - | 186.3±11.0 a | 32 | 269.2±13.0 b | 40 | |
| Tyr | - | - | 380.0±11.0 a | 32 | - | - | 305.0±8.0 a | 32 |
| Leu | 410.0±14.0 a | 40 | - | - | 196.1±4.0 a | 40 | 250.0±6.0 b | 56 |
a, b: Different letters in a same column indicate the data are significantly different as evaluated by Tukey test (p<0.01). ‘-’means the results are not detectable. The abbreviations are phenylalanine (Phe), tyrosine (Tyr), leucine (Leu), phenylalanine (PAL), tyrosine ammonia-lyase (TAL), trans-cinnamate 4-hydroxylase (C4H) and 4-coumarate-CoA ligase (4CL).