Table 4.
Marker mining on 23 genome sequence assembly scaffolds bearing 24 markers linked to 11 key genes of agronomic traits of interest by sequence alignments among 10 sequenced cultivars of Lupinus angustifolius a
| Agronomic traits | Name of markers | Distance between marker and target gene (cM) | Reference | Scaffold identified | Scaffold size (bp) | Number of SNP markers from scaffold sequence alignment | Number of InDel markers from scaffold sequence alignment |
|---|---|---|---|---|---|---|---|
| Disease resistance gene PhtjR | DAFWA6895 | 0 | [38] | Scaffold84773 | 33,448 | 489 | 101 |
| Disease resistance gene PhtjR | PhtjM1 | 1.3 | [44] | scaffold70674 | 11,068 | 102 | 39 |
| Disease resistance gene PhtjR | PhtjM4 | 1.1 | [44] | scaffold16849 | 40,716 | 526 | 259 |
| Disease resistance gene PhtjR | PhtjM6 | 1.9 | [44] | scaffold2572 | 55,753 | 808 | 263 |
| Disease resistance gene PhtjR | PhtjM7 | 1.1 | [44] | scaffold57606 | 13,893 | 188 | 62 |
| Disease resistance gene Lanr1 | DAFWA5820 | 0 | [38] | scaffold 31581 | 15,706 | 225 | 33 |
| Disease resistance gene Lanr1 | AntjM1 | 3.5 | [22] | scaffold83350 | 11,407 | 74 | 35 |
| Disease resistance gene Lanr1 | AntjM2 | 2.3 | [14] | scaffold2992 | 33,979 | 341 | 188 |
| Disease resistance gene Lanr1 | AnSeq3 | 0.9 | [37] | Scaffold33942 | 64,039 | 716 | 138 |
| Disease resistance gene Lanr1 | AnSeq4 | 0.9 | [37] | Scaffold31346 | 33,727 | 221 | 158 |
| Seed coat colour | DAFWA6428 | 0 | [38] | scaffold11676 | 22,481 | 588 | 154 |
| Seed coat colour | DAFWA4544 | 0 | [38] | scaffold13708 | 44,176 | 821 | 81 |
| Disease resistance gene AnMan | AnManM1 | 5.0 | [16] | scaffold36514 | 50,220 | 311 | 213 |
| Disease resistance gene Phr1 | Ph258M1 | 5.7 | [21] | scaffold84752 | 21,471 | 292 | 94 |
| Disease resistance gene Phr1 | Ph258M2 | 2.1 | [21] | scaffold16252 | 15,559 | 212 | 25 |
| Resistance gene against lupin rust disease | RustM1 | Unknown | Unpublished | scaffold15347 | 42,210 | 578 | 25 |
| Early flowering gene Ku | KuH | 0 | [25] | scaffold21489 | 30,923 | 676 | 23 |
| Soft-seed coat gene mollis | MoA, MoLi | 0 | [23, 30] | scaffold75616 | 14,783 | 63 | 16 |
| Pod-non-shattering le | LeLi | 6.0 | [29] | scaffold87978 | 9,909 | 59 | 17 |
| Pod-non-shattering gene le | LeM2 | 1.3 | [24] | scaffold79908 | 20,738 | 103 | 22 |
| Pod-non-shattering gene tardus | TaM1 | 2.1 | [26] | scaffold15347 | 21,529 | 578 | 25 |
| Pod-non-shattering gene tardus | TaLi | 1.4 | [27] | scaffold36274 | 8,191 | 62 | 4 |
| Low alkaloid gene iucundus | IucLi | 0.9 | [28] | scaffold30160 | 20,677 | 667 | 22 |
| Average scaffold size and marker numbers | 27,687 | 378 | 87 | ||||