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. 2015 Sep 2;10(9):e0137461. doi: 10.1371/journal.pone.0137461

Fig 1. Enrichment and detection of small non-coding miRNA from sciatic nerve axoplasm.

Fig 1

A. Extended RT-PCR of mRNAs from sciatic nerve axoplasm using either by the conventional whole tissue lysate method or a mechanical squeezing procedure. Note that neither cell body restricted (MAP2, H1F0) nor glial (GFAP, ErbB3) mRNAs were amplified from sciatic nerve cDNAs reverse-transcribed from the squeezed axoplasmic RNAs. B. Size and frequency distribution of regulatory and small non-coding RNAs in uninjured sciatic nerve. C. A size histogram of mapped small RNAs. >40% of small RNAs were mapped to miRNAs, while 30% of small RNA sequences could not be annotated.