Table 3. qPCR analysis of human podocyte gene expression: Verification of selected microarray results.

Gene Symbol	Gene Title	PAN+MeOH vs. MeOH			PAN+EV vs. PAN+MeOH			Function
		FC	p-value	FC Array*	FC	p-value	FC Array*
COL4A3	Collagen, type IV, alpha 3	-1.3	ns	-4.8	2.6	0.002	3.3	extracellular matrix composition
DCDC2	Doublecortin domain containing 2	-3.9	0.001	-6.6	2.4	0.004	2.3	cytoskeletal organization / stability, migration
FN1	Fibronectin 1	-2.0	0.03	-1.8	-1.0	ns	1.5	adhesion, migration
LPXN	Leupaxin	5.6	0.01	2.4	-2.2	0.03	-2.2	adhesion, migration
MMP24	Matrix metallopeptidase 24	-1.6	0.01	-2.4	3.0	ns	1.6	migration
MYLIP	Myosin regulatory light chain interacting protein	15.7	0.01	5.3	-2.3	0.04	-2.6	cytoskeletal organization / stability
MYO5C	Myosin VC	-4.1	0.002	-5.8	2.8	0.009	2.3	cytoskeletal organization / stability
NRG1	Neuregulin 1	-1.6	ns	-3.2	1.2	ns	2.1	adhesion
PCDH10	Protocadherin 10	71.1	0.009	13.9	-4.6	0.01	-4.9	adhesion
PCDH17	Protocadherin 17	371.4	0.005	17.7	-12.0	0.006	-10.5	adhesion
PCDH9	Protocadherin 9	32.7	0.02	9.5	-6.7	0.02	-5.7	adhesion
SYNPO2	Synaptopodin 2	1.9	ns	2.1	-2.4	0.04	-3.0	cytoskeletal organization / stability
TGFB1I1	Transforming growth factor beta 1 induced transcript 1	-1.7	0.05	-5.1	2.9	0.008	2.0	adhesion
THBS1	Thrombospondin 1	-10.8	0.0003	-13.5	2.5	0.03	2.5	adhesion
TUBA1A	Tubulin, alpha 1a	-3.8	0.0001	-2.9	1.6	0.03	2.0	cytoskeletal organization / stability
TUBB	Tubulin, beta class I	-3.9	0.0007	-5.5	2.2	0.004	2.0	cytoskeletal organization / stability
TUBB2B	tubulin, beta 2B class 2b	-13.5	0.00008	-19.0	5.8	0.003	4.2	cytoskeletal organization / stability

PAN = puromycin aminonucleoside. EV = everolimus. MeOH = methanol, solvent for EV. p = p-value. FC = fold change.

* = p < 0.05.