Fig 7. Flow cytometry analysis of the sperm binding capacity of Alexa Fluor 488-conjugated RSVP20.
Cytometry analysis of the effect of sperm incubation with increasing concentration of Alexa-conjugated RSVP20: A) 3 μg; B) 7.5 μg; C) 15 μg per 4 x 107 cells. D) Plots showing the number of cells linked with protein (events) and the different fluorescence intensity (FL1 Log) for increasing concentrations of Alexa-conjugated RSVP20.