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. 2015 Sep 2;10(9):e0137003. doi: 10.1371/journal.pone.0137003

Fig 3. Association between high-grade NMIBC tumour methylation and gene expression.

Fig 3

Quantitative RT-PCR analyses of HOXA9, ISL1, SPAG6 and ZNF154 transcript expression in individual high-grade tumours (HG). Expression is reported relative to the mean of three normal bladder controls (C, triangles), where the mean value is expressed as equal to 100%. Filled and unfilled circles denote methylated or unmethylated tumours, and represent the mean value from two independent experiments performed in triplicate. The horizontal bars within the control column represent the mean of the controls, a 3-fold reduction beneath this lies the double-headed arrow in each gene plot, representing the cut-off for significantly reduced expression in tumours relative to controls.