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. 2015 Sep 2;10(9):e0136879. doi: 10.1371/journal.pone.0136879

Fig 5. Activities of Rpo41 variants on pre-melted promoter-containing DNA.

Fig 5

(A). The duplex DNA template contains a 14S rRNA promoter, with non-complementary sequences from positions-4 to +2. (B) (1) [γ-32P]ATP labeled transcripts on the above template (1 μM) using Rpo41 (0.5 μM wild-type or a variant) in the absence or presence of Mtf1 (2.5 μM) were visualized on a polyacrylamide denaturing gel. (2) Same as (1), except that transcripts are labeled with [γ-32P]ATP, [γ-32P]GTP or [α-32P]ATP. C) The intensities of run-off transcripts in B (1) quantified using Quantity One software.