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. 2015 Sep 2;10(9):e0136797. doi: 10.1371/journal.pone.0136797

Fig 2. Downregulation of MT1-MMP blocks TIMP-2 activation of AKT in MT1-MMP expressing MDA-MB-435 cells.

Fig 2

Western blotting analysis of AKT activation (p-AKT) and MT1-MMP expression in MDA-MB-435 cells transiently transfected with MT1-MMP siRNA (siMT1-MMP) or control, scrambled siRNA (siControl), and incubated with TIMP-2 (100 ng/ml) for 15 min. Total AKT and β-tubulin (TUB) are shown as loading controls. The lower panel shows the densitometric analysis of the MT1-MMP and p-AKT bands normalized to the corresponding TUB and AKT controls, respectively. *, p ≤ 0.05; + TIMP-2 vs. the corresponding – TIMP-2 sample. This experiment was repeated twice with comparable results.