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. 2015 Sep 2;10(9):e0136797. doi: 10.1371/journal.pone.0136797

Fig 3. TIMP-2 induces AKT activation in a dose- and time-dependent manner.

Fig 3

Western blotting analysis of AKT activation (pAKT) in MT1-MMP Tet-Off MCF-7 cells incubated with (A) TIMP-2 (100 ng/ml) for the indicated times or (B) with the indicated concentrations of TIMP-2 for 15 min. The cells shown in panel A were grown in the absence of DOX; the cells shown in panel B were grown either in the presence (-MT) or in the absence (+MT) of DOX. Total AKT is shown as a loading control. C and D. Densitometric analysis. *, p ≤ 0.05, vs. time 0; #, p ≤ 0.05, + MT1-MMP vs. the corresponding – MT1-MMP sample. These experiments were repeated twice with similar results.