Fig 3. Lipophil granule secretion.

(A). Field of lipophil granules (large orange spheres) near a clump of algae and algae debris (yellow-green) prior to and following granule secretion (cyan circles; time interval 308 ms). Secreted granules (yellow arrows) enlarged and became greener due to uptake of FM1-43 dye. (B-D). Sequential images (61.5 ms intervals) showing secretion of individual granules (yellow arrows). Granules appeared as red/green doublets during secretion because the granules were moving and red/green channels were captured sequentially. After secretion, the contents of the granule began to disperse. Lipophil granules were stained with Lysotracker Red (visible in both red and green channels). FM1-43 (green) was added to the seawater to stain the contents of the secreted granules. Sequential two-channel, single focal plane time series collected with a spinning disk confocal microscope. Scale: A- 20 μm, B- D 10 μm.