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. 2015 Sep 2;10(9):e0136798. doi: 10.1371/journal.pone.0136798

Fig 1. Measuring allelic mRNA occupancy on polysomes.

Fig 1

The flowchart depicts the strategy used to fractionate cellular components for RNA analysis and to determine Allelic Expression Imbalance (AEI), defined as a ratio of reference allele (REF) to alternative allele (ALT). Chromatographic peaks obtained from SNaPshot [25] represent relative allelic abundance in gDNA and mRNA (as cDNA). Significant deviation from unity (after normalization to allelic ratios of gDNA or plasmid DNA) indicates unequal polysomal occupancy of allelic mRNA. RNPs represent cytosolic mRNA either polysome-free or bound to ribonucleotide particles.