Table 4. Genes expressing RNA isoforms with differential loading onto polysomes, and displaying different allelic RNA ratios in cytosolic and polysomal extracts.
Targets with > 2 fold difference in allelic ratio between cytosol and polysome were analyzed for isoform differences. Results show both enrichment and depletion of isoforms on polysomes.
| % Isoform* | Allelic ratio | ||||||
|---|---|---|---|---|---|---|---|
| Isoform ID | Gene | Cytosol | Polysome | Cytosol | Polysome | Transcript Type | Location |
| ENST00000226798.4 | FRG1 a | 67 | 31 | 1 | 2.7 | Protein coding | exonic |
| ENST00000253413.5 | ATP6V1E1 a | 91 | 63 | 2.8 | 1.2 | Protein coding | 5' UTR/ 3' UTR |
| ENST00000254719.5 | RPA1 a | 72 | 12 | 4.2 | 1 | Protein coding | exonic/3' UTR |
| ENST00000285243.6 | ANKRD40 b | 60 | 83 | 2.4 | 1 | Protein coding | 3' UTR |
| ENST00000292476.5 | CPSF4 a | 99 | 73 | 2.6 | 1.1 | Protein coding | intronic |
| ENST00000292538.4 | CUX1 b | 38 | 61 | 1 | 2.2 | Protein coding | exonic |
| ENST00000312419.3 | POLD4 a | 90 | 63 | 2.9 | 1.3 | Protein coding | 3' UTR |
| ENST00000319004.5 | GEMIN4 a | 91 | 75 | 1.2 | 3.8 | Protein coding | exonic |
| ENST00000338754.4 | TPST2 a | 76 | 12 | 2.8 | 1.2 | Protein coding | exonic |
| ENST00000357984.3 | TMUB2 b | 28 | 57 | 3.9 | 1.3 | Protein coding | 3' UTR |
| ENST00000361439.4 | NSMCE1 b | 57 | 91 | 2.3 | 1 | Protein coding | exonic/ 3' UTR |
| ENST00000361445.4 | MTOR a | 82 | 57 | 3 | 1.1 | Protein coding | exonic |
| ENST00000368324.4 | SYT11 a | 100 | 55 | 3.1 | 1.3 | Protein coding | 5' UTR |
| ENST00000372235.3 | TMEM53 b | 27 | 59 | 2.8 | 1.1 | Protein coding | 3' UTR |
| ENST00000373616.5 | MTCH1 a | 87 | 57 | 3 | 1.3 | Protein coding | 3' UTR |
| ENST00000398110.2 | TPST2 b | 24 | 88 | 2.8 | 1.2 | Protein coding | exonic |
| ENST00000440410.1 | DLD a | 55 | 30 | 2.5 | 1.1 | Protein coding | exonic |
| ENST00000444411.1 | MMS19 b | 39 | 62 | 2.2 | 1.7 | Protein coding | exonic |
| ENST00000447062.2 | WDTC1 a | 51 | 20 | 2.4 | 1 | Nonsense mediated decay | 3' UTR |
| ENST00000483469.1 | GET4 a | 52 | 32 | 2.3 | 1.1 | Retained intron | exonic |
| ENST00000483996.1 | SUN1 b | 51 | 70 | 2.3 | 1.1 | Retained intron | exonic |
| ENST00000491660.1 | SERP1 b | 27 | 56 | 1.1 | 2.8 | Protein coding | exonic |
| ENST00000492590.1 | FHIT a | 100 | 74 | 3.2 | 1.1 | Protein coding | exonic |
| ENST00000492955.1 | LY75 a | 55 | 30 | 4 | 1.8 | Processed transcript | exonic/ intronic |
| ENST00000518016.1 | FBXO16 b | 3 | 48 | 2.6 | 1.1 | Retained intron | exonic |
| ENST00000529725.1 | SIPA1 a | 55 | 33 | 2.8 | 1.3 | Retained intron | exonic |
| ENST00000534474.2 | MPV17L2 a | 65 | 39 | 3.4 | 1.1 | Novel Protein coding | exonic |
| ENST00000540523.1 | STAM b | 52 | 71 | 1 | 2.6 | Protein coding | exonic |
| ENST00000570487.1 | RABEP1 b | 47 | 73 | 2.7 | 1.1 | Protein coding | exonic |
| ENST00000587040.1 | SYNRG b | 38 | 58 | 2.6 | 1.5 | Processed transcript | exonic |
aRNA isoforms depleted on polysomes.
bRNA isoforms enriched on polysomes.
* Percentage of each isoform in the pool of all isoforms for given gene.