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. 2015 Apr 13;6(17):15008–15021. doi: 10.18632/oncotarget.3634

FIGURE 2. PARP-1 activity is required for Snail upregulation in doxo-treated MDA-MB-231 cells.

FIGURE 2

MDA-MB-231 cells were treated with 1 μM doxo, 1 μM doxo plus 0.5 μM ABT-888, 0.5 μM ABT-888 alone at the indicated times. A. PAR, PARP-1 (detected with mAb clone F1–23 Enzo Life Sciences) and Snail levels were assessed by Western blot analysis B. Graph shows the average densitometry of Snail values normalized to actin, considering Snail level in untreated cells as 1.0. Data represent mean + SEM of three independent experiments. *P < 0.05, **P < 0.01 by Student's t-test. C. Expression levels of SNAI1 mRNA were assessed by Real-Time PCR after 2 h (white bars), 7 h (light gray bars) and 24 h (dark gray bars) of treatment and compared to untreated cells (black bar) considered as 1.0. Data represent mean + SEM of at least five independent experiments performed in triplicates. *P < 0.05, **P < 0.01 by Students t-test. D. Snail protein level was assessed by Western blot in MDA-MB-231 cells silenced for PARP-1 after treatment with 1 μM doxo for 7 h. E. Graph shows the average densitometry of Snail values normalized to actin, considering Snail level in untreated cells as 1.0. Data represent mean + SEM of three independent experiments. *P < 0.05 by Student's t-test. F. Levels of SNAI1mRNA in MDA-MB-231 cells silenced for PARP-1 (dark gray bars) after treatment with 1 μM doxo for 7 h in relation with cells transfected with siCT (light gray bars). Data represent mean + SEM of three independent experiments performed in triplicates. **P < 0.01 by Student's t-test.