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. 2015 Apr 13;6(17):15008–15021. doi: 10.18632/oncotarget.3634

FIGURE 4. PARP-1 inhibition/depletion antagonizes doxo-induced downregulation of PTEN in MDA-MB-231 cells and decreases Akt activity.

FIGURE 4

A. Expression levels of PTEN after treatment with 1 μM doxo, 1 μM doxo plus 0.5 μM ABT-888 or ABT-888 for 7 h (light gray bars) or 24 h (dark gray bars) in relation to untreated cells (white bar), considered as 1.0. Data represent mean+SEM of at least five independent experiments performed in triplicates. *P < 0.05, **P < 0.01 by Student's t-test. B. Levels of Akt, phospho-Akt, PTEN and γH2AX protein were measured by Western blot analyses in MDA-MB-231 cells treated for 24 h with 1 μM doxo and/or 0.5 μM ABT-888 as indicated. C. Expression levels of PTEN after treatment of siCT and siPARP-1 MDA-MB-231 cells with 1 μM doxo for 7 h (light gray bars) or 24 h (dark gray bars) in relation to untreated siCT and siPARP-1 cells (white bars), considered as 1.0. Data represent mean+SEM of three independent experiments performed in triplicates. *P < 0.05 by Student's t-test. D. Levels of PARP-1, Akt, phospho-Akt, and PTEN protein were measured by Western blot analyses in siCT and siPARP-1 MDA-MB-231 cells treated for 24 h with 1 μM doxo.