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. 2015 Apr 13;6(17):15008–15021. doi: 10.18632/oncotarget.3634

FIGURE 5. Snail knockdown sensitizes MDA-MB-231 cells to doxo-induced apoptosis and allows recovery of PTEN expression.

FIGURE 5

A. Levels of Snail protein were measured by Western blot analyses in shCT and shSnail MDA-MB-231 cells treated for 24 h with 1 μM doxo B. Annexin V positive cells were counted in the right upper and lower squares. The diagram reports the percentage of Annexin V positive cells in untreated shCT and shSnail cells (black bars) and after treatment of shCT (white bars) and shSnail cells (light gray bars) with 1 μM doxo at the indicated times in relation to total cells. Data represented are the mean+SEM of three independent experiments performed in duplicates. Comparisons were made with ANOVA/Turkey's test. *P < 0.05, **P < 0.01 compared to cells treated with doxo at 24 h, 48 h respectively. C. Expression levels of PTEN after treatment of shCT and shSnail MDA-MB-231 cells with 1 μM doxo for 7 h (light gray bars) or 24 h (dark gray bars) in relation to untreated shCT and shSnail cells (white bars), considered as 1.0. Data represent mean+SEM of three independent experiments performed in triplicates. *P < 0.05 by Student's t-test.