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. 2015 Apr 2;6(17):15022–15034. doi: 10.18632/oncotarget.3609

Figure 2. Fibulin-5 silencing is correlated with activation of the Wnt/β-catenin pathway in lung cancer.

Figure 2

A. Heatmap for comparing fibulin-5 and MMP-7 mRNA expression in the TCGA lung cancer (LUNG) RNAseq (IlluminaHiSeq; N = 1081) dataset. B. Heatmap for comparing fibulin-5 and c-Myc mRNA expression in the TCGA lung cancer (LUNG) RNAseq (IlluminaHiSeq; N = 1081) dataset. C. Analysis of fibulin-5 and β-catenin expression in NSCLC by immunostaining. Pictures show an example tumor that is positive for fibulin-5 but negative for nuclear β-catenin staining, and an example tumor that is negative for fibulin-5 but positive for nuclear β-catenin staining. Arrows in the enlarged field indicate example cells with nuclear β-catenin staining. Scale bar: 50 μm. D. Summary of fibulin-5 and nuclear β-catenin staining results in 99 NSCLC samples. The inverse correlation between fibulin-5 expression and nuclear β-catenin expression was significant (P = 0.0073, two-tailed χ2 test). E. Top, summary of nuclear β-catenin expression and fibulin-5 promoter methylation in an independent set of 30 pairs of lung tumors and matched pathologically normal lung tissues. Bottom, correlation of nuclear β-catenin expression and fibulin-5 promoter methylation in lung tumors (P = 0.0235, two-tailed χ2 test).