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. 2015 Apr 2;6(17):15022–15034. doi: 10.18632/oncotarget.3609

Figure 3. Fibulin-5 inhibits β-catenin nuclear translocation and TCF-4 activity.

Figure 3

A. Western blotting of β-catenin in nuclear fractions isolated from H1299 and A549 cells at 24 hr after transfection with fibulin-5 or the control empty vector. B. H1752 cells transfected with fibulin-5 or control siRNA were analyzed for β-catenin by immunostaining 48 hr after transfection. Left, representative pictures of β-catenin immunostaining. Scale bar, 5 μm. Right, quantification of cells with nuclear β-catenin (P = 0.0002, Student's t test). C. A549 and H1299 cells were transfected with fibulin-5 along with the TCF-4 reporter pTOPFlash (OT) or the control inactive reporter pFOPFlash (OF). Normalized luciferase activities were determined 24 hr after transfection. The activity of the OF was defined as 1.0. D. The effects of fibulin-5 on TCF-4 reporter activities induced by WT or mutant β-catenin (ΔN). ΔN: the mutant β-catenin with amino-terminal 45 amino acids deleted. Reporter assays were performed as in (C).