Skip to main content
. 2015 Apr 10;6(17):15111–15121. doi: 10.18632/oncotarget.3615

Figure 4. HHT promotes proteasome-dependent degradation of p-eIF4E via enhancing its SUMOylation.

Figure 4

A. Western blot analysis of p-eIF4E in the supernatant (S) and pellet (P) fractions of lysates of THP-1 cells treated with 100 ng/ml HHT for the indicated time points. GAPDH was used as loading control. B. Western blot analysis of p-eIF4E and total-eIF4E (t-eIF4E) in whole cell lysates from THP-1 cells treated with the indicated concentrations of HHT and MG132 (2 μg/ml) for 24 h. C. and D. HEK 293 cells transfected with FLAG-eIF4E or FLAG-eIF4E-209 plasmids were treated with or without HHT for 12 h. After harvesting, cells were lysed, followed by immunoprecipitation with anti-FLAG M2 beads and subsequent western blot analysis with either anti-SUMO2/3 (C) or anti-SUMO1 (D). E. Time course analysis of HHT-induced UBC9 conjugation to p-eIF4E in THP-1 cells. THP-1 cells were treated with HHT (100 ng/ml) for the indicated time points.